Difference between revisions of "Part:BBa K2719003:Experience"
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===Applications of BBa_K2719003=== | ===Applications of BBa_K2719003=== | ||
| − | This part was cloned on pSB1C3 by restriction with EcoRI and PstI and transformed into E.coli DH5a, obtaining white colonies (see figure 1). | + | <p>This part was cloned on pSB1C3 by restriction with EcoRI and PstI and transformed into <i>E.coli</i> DH5a, obtaining white colonies (see figure 1).</p> |
| + | [[file:T--TecCEM--OPGSTColonies.png|500px]] | ||
| + | <p><i>Figure 1.</i> Colonies transformed with optimized GST.</p> | ||
| − | Latter it has extracted and run on an agarose gel electrophoresis (see figure 2). | + | <p>Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).</p> |
| − | Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3). | + | <p>Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).</p> |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:44, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2719003
This part was cloned on pSB1C3 by restriction with EcoRI and PstI and transformed into E.coli DH5a, obtaining white colonies (see figure 1).
Figure 1. Colonies transformed with optimized GST.
Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).
Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).
User Reviews
UNIQ61b3fd76f7f65854-partinfo-00000000-QINU UNIQ61b3fd76f7f65854-partinfo-00000001-QINU