Difference between revisions of "Part:BBa K2818002"
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===Usage and Biology===
 
===Usage and Biology===
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As mentioned above, the dPspCas13b is the catalytically inactive version of Type VI RNA-targeting CRISPR-associated protein 13b, an RNA-guided ribonuclease derived from <html><i>Prevotella sep. P5-125</i></html>and it acts as the RNA-targeting scaffold to bind to specific RNA target sequence. Such a binding action is mediated by a single guide RNA, the sequence of which greatly affects the binding efficiency of dCas protein onto the target and ultimately the functionality of the fused protein. <br><br>
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The other
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Revision as of 16:33, 17 October 2018


Cas13b-NES-ADAR

dPspCas13b-ADAR2DD(E488Q) is a fusion protein that catalyzes the hydrolytic deamination of adenosine to form inosine in RNA molecules when used in conjunction with a guide RNA. Although there are preferred motifs, no Protospacer Adjacent Motif (PAM) is required. It is an optimized construct obtained developed by Zhang Feng's lab (Feng et. al., 2017) to mediate efficient adenosine to inosine on specific positions on mRNA target, which can be programmed by a specific guide RNA. This construct is submitted as it is currently not in the iGEM registry and it acts as an important basis for comparison when we characterize our new part this year (BBa_K2818001).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1787
    Illegal BamHI site found at 839
    Illegal BamHI site found at 3310
    Illegal XhoI site found at 4011
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1520
    Illegal AgeI site found at 2396
  • 1000
    COMPATIBLE WITH RFC[1000]