Difference between revisions of "Part:BBa K2752012"

Revision as of 15:38, 17 October 2018

Uox

This urate oxidase is novel mammalian uricase protein, containing some human uricase chimeric protein having an amino acid sequence of the uricase activity of mammalian origin and mutant proteins. The amino acid sequence of the 241-304th of the urate oxidase is derived from human, and the amino acid sequence of the 1-240th is derived from the amino acid sequence of the uricase of pigs and dogs.

Sequence and Features

Characterization

A plasmid containing a urate oxidase sequence was introduced into the BL21 strain, and IPTG was used as an inducer to induce expression of urate oxidase. After expression, the cells were disrupted by sonication and the supernatant was taken. The supernatant (extracellular protein) of the first centrifugation and the supernatant (intracellular protein) after sonication were subjected to polyacrylamide gel electrophoresis. The electrophoresis results are shown in Figure 1.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 55
Illegal AgeI site found at 406
Illegal AgeI site found at 877
1000
COMPATIBLE WITH RFC[1000]

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 <span class='h3bb'>Sequence and Features</span>
+===Characterization===
+A plasmid containing a urate oxidase sequence was introduced into the BL21 strain, and IPTG was used as an inducer to induce expression of urate oxidase. After expression, the cells were disrupted by sonication and the supernatant was taken. The supernatant (extracellular protein) of the first centrifugation and the supernatant (intracellular protein) after sonication were subjected to polyacrylamide gel electrophoresis. The electrophoresis results are shown in Figure 1.
 <partinfo>BBa_K2752012 SequenceAndFeatures</partinfo>