Difference between revisions of "Part:BBa K2632007"

Revision as of 15:18, 17 October 2018

A377D mutant of full length Gasdermin D

Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that N-terminal of Gasdermin D (BBa K2632003) acts as a effector of pyroptosis. Full length Gasdermin D is cleaved by Caspase 1 then release the PFD (pore-forming domain) which can oligomerize on the cell membrane. Formation of pore causes cell swelling, rupture of the membrane and massive leakage of cytosolic contents. A377D mutation of full length Gasdermin D reduces the cell toxicity.

Reference

Ding J, Wang K, Liu W, et al. Pore-forming activity and structural autoinhibition of the gasdermin family[J]. Nature, 2016, 535(7610):111.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1204
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 883

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-Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that  N-terminal of Gasdermin D <a href="https://parts.igem.org/Part:BBa_K2632003">(BBa K2632003)</a> acts as a effector of pyroptosis. Full length Gasdermin D is cleaved by Caspase 1 then release the PFD(pore-forming domain) which can oligomerize on the cell membrane. Formation of pore causes cell swelling, rupture of the membrane and massive leakage of cytosolic contents. A377D mutation of full length Gasdermin D reduces the cell toxicity.
+Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that  N-terminal of Gasdermin D <a href="https://parts.igem.org/Part:BBa_K2632003">(BBa K2632003)</a> acts as a effector of pyroptosis. Full length Gasdermin D is cleaved by Caspase 1 then release the PFD (pore-forming domain) which can oligomerize on the cell membrane. Formation of pore causes cell swelling, rupture of the membrane and massive leakage of cytosolic contents. A377D mutation of full length Gasdermin D reduces the cell toxicity.
 <h3>Reference</h3>