Difference between revisions of "Part:BBa K2533046"
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<h1>'''Usage and biology'''</h1>
 
<h1>'''Usage and biology'''</h1>
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dld refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. With the overexpression of dld, Shewanella could utilize D-lactate more efficiently, which brings more electricity being produced.
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lldP refers to L-lactate permease, helping the lactate transported out of the cell. With this gene, Rhodopseudomonas palustris could transport lactate out of the cell more efficiently, which brings Shewanella more carbon sources.
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<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
 
This is one section for lactate utilization part.
 
This is one section for lactate utilization part.
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<h2>Electrogenesis</h2>
 
<h2>Electrogenesis</h2>
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By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity.
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By detecting the production of lactate after expressing protein, we might find out whether mleS could effectively help Rhodopseudomonas palustris to produce more lactate.
 
[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.]]
 
[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.]]
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It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty.  
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It could be demonstrated that targeted genes could be expressed in the engineered cells. More lactate has been transported out of the cell by engineered bacteria, which helps to provide more carbon sources for Shewanella.  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 15:01, 17 October 2018

transport of lactic acid

RBS-mleS

transport lactate

Usage and biology

lldP refers to L-lactate permease, helping the lactate transported out of the cell. With this gene, Rhodopseudomonas palustris could transport lactate out of the cell more efficiently, which brings Shewanella more carbon sources.

Characterization

This is one section for lactate utilization part.

Figure1:RBS-lldP

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.

Figure2:Verification of successful transformation of pSB1C3-RBS-lldP

Our target genes are 1713bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.

Electrogenesis

By detecting the production of lactate after expressing protein, we might find out whether mleS could effectively help Rhodopseudomonas palustris to produce more lactate.

Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.

It could be demonstrated that targeted genes could be expressed in the engineered cells. More lactate has been transported out of the cell by engineered bacteria, which helps to provide more carbon sources for Shewanella.