Difference between revisions of "Part:BBa K2533045"
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<partinfo>BBa_K2533045 short</partinfo>
 
<partinfo>BBa_K2533045 short</partinfo>
  
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produce latic acid
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produce lactate
  
 
<h1>'''Usage and biology'''</h1>
 
<h1>'''Usage and biology'''</h1>
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dld refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. With the overexpression of dld, Shewanella could utilize D-lactate more efficiently, which brings more electricity being produced.
+
mleS refers to malate dehydrogenase, enhancing the conversion of malic acid to L-lactate. Rhodopseudomonas palustris could utilize lactate more efficiently, which brings Shewanella more carbon sources.
  
 
<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
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<h2>Electrogenesis</h2>
 
<h2>Electrogenesis</h2>
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By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity.
+
By detecting the production of lactate after expressing protein, we might find out whether mleS could effectively help Rhodopseudomonas palustris to produce more lactate.
 
[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.]]
 
[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.]]
−
It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty.  
+
It could be demonstrated that targeted genes could be expressed in the engineered cells. More lactate has been produced by engineered bacteria, which helps to produce more lactate.  
  
 
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Revision as of 14:45, 17 October 2018


RBS-mleS

produce lactate

Usage and biology

mleS refers to malate dehydrogenase, enhancing the conversion of malic acid to L-lactate. Rhodopseudomonas palustris could utilize lactate more efficiently, which brings Shewanella more carbon sources.

Characterization

This is one section for lactate utilization part.

Figure1:RBS-mles

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.

Figure2:Verification of successful transformation of pSB1C3-RBS-mleS

Our target genes are 1969bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.

Electrogenesis

By detecting the production of lactate after expressing protein, we might find out whether mleS could effectively help Rhodopseudomonas palustris to produce more lactate.

Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.

It could be demonstrated that targeted genes could be expressed in the engineered cells. More lactate has been produced by engineered bacteria, which helps to produce more lactate.