Difference between revisions of "Part:BBa K2533003:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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None.
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LdhDARSdR is the codon-optimized version of ldhDnARSdR, and ldhDARSdR is ldhD (lactate dehydrogenase gene) with D176A/I177R/F178S/N180R. LldP ​​protein gene is used to transport the lactate out of the cell. We successfully construct genes ldhDARSdR-lldp using molecular biology experiment methods.
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===Source===
 
===Source===

Revision as of 14:29, 17 October 2018


RBS-ldhDARSdR-RBS-lldP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1461
    Illegal AgeI site found at 1924
    Illegal AgeI site found at 2362
    Illegal AgeI site found at 2521
    Illegal AgeI site found at 2533
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 764
    Illegal BsaI.rc site found at 1035


Design Notes

LdhDARSdR is the codon-optimized version of ldhDnARSdR, and ldhDARSdR is ldhD (lactate dehydrogenase gene) with D176A/I177R/F178S/N180R. LldP ​​protein gene is used to transport the lactate out of the cell. We successfully construct genes ldhDARSdR-lldp using molecular biology experiment methods.

Source

From Lactobacillus.


References