Difference between revisions of "Part:BBa K2595003"

Latest revision as of 14:29, 17 October 2018

MicC

The small RNA MicC is involved in regulation of the expression of the gene ompC at the post transcriptional level. Overexpression of MicC causes a 60% reduction in the expression of ompC. On the other hand, a deletion of MicC leads to 1.5-2-fold decrease in the expression of ompC. This results from 22 nucleotides at 5’ end of the MicC, which are able to base pair with a sequence located upstream of Ribosome Binding Site in the ompC mRNA. This represses ompC expression by causing inhibition of the binding of the 30S ribosomal mRNA. Hfq is required for the proper functioning of MicC. If MicC is overexpressed, it causes a reduction in swarming motility. Expression of MicC is induced by growth at low temperature or on minimal media. It is also induced by the presence of carbapenems and cephalosporins, which are members of the β-lactam class of antibiotics. It can also be induced by the overexpression of RseA, which causes depletion of σE.

Usage and Biology

This synthetic version of MicC follow the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, MicC can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2595003 short</partinfo>
--
+The small RNA MicC is involved in regulation of the expression of the gene ompC at the post transcriptional level.  Overexpression of MicC causes a 60% reduction in the expression of ompC. On the other hand, a deletion of MicC leads to 1.5-2-fold decrease in the expression of ompC.
+This results from 22 nucleotides at 5’ end of the MicC, which are able to base pair with a sequence located upstream of Ribosome Binding Site in the ompC mRNA. This represses ompC expression by causing inhibition of the binding of the 30S ribosomal mRNA.
+Hfq is required for the proper functioning of MicC. If MicC is overexpressed, it causes a reduction in swarming motility.
+Expression of MicC is induced by growth at low temperature or on minimal media. It is also induced by the presence of carbapenems and cephalosporins, which are members of the β-lactam class of antibiotics. It can also be induced by the overexpression of RseA, which causes depletion of σE.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This synthetic version of MicC follow the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, MicC can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence.  Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.
 <!-- -->