Difference between revisions of "Part:BBa K2595001"

Latest revision as of 14:18, 17 October 2018

DsrA

DsrA is a non-coding RNA that has roles in both transcription and translation. It can overcome transcriptional silencing by association with H-NS protein and it is also involved in the translation of stress sigma factor RpoS. (Sledjeski and Gottesman, 1995) DsrA folds into a secondary structure comprising of three hairpins by which the second binds to Hfq. (Majdalani et al., 1998) There is experimental evidence that DsrA has roles in several molecular functions including 4 iron/4 sulphur cluster binding, haem binding, hydrogen sulfite reductase activity, and metal ion binding. (Uniprot.org, 2018) The first stem loop of DsrA is important in the translation of RpoS, the sequence is complementary to the RpoS upstream messenger RNA, but not for action of H-NS. The second stem loop is required for anti-silencing and the third stem loop acts as a terminator for transcription. (Wu et al., 2017)

Usage and Biology

This synthetic version of DsrA follows the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, DsrA can regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-This synthetic version of DsrA follows the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, DsrA can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence.  Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.
+This synthetic version of DsrA follows the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, DsrA can regulate the expression of the gene that contains the complementary regions for its targeting sequence.  Ligation with the desired targeting sequence does not create any scar in the sRNA sequence.
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