Difference between revisions of "Part:BBa K2558003"

 
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<partinfo>BBa_K2558003 short</partinfo>
 
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Cas9 is the main protein of the CRISPR/Cas system II of Streptococcus pyogenes. CRISPR systems protect bacteria and archaea from phages by recognizing and cleaving of invading phage DNA. In practice a short gRNA is used as guide and bind the Cas9 protein to its complementary DNA strand. Cas9 protein would cleave the DNA strand at the binding site.  
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Cas9 is the main protein of the CRISPR/Cas system II of Streptococcus pyogenes. CRISPR systems protect bacteria and archaea from phages by recognizing and cleaving of invading phage DNA. In practice, a short gRNA is used as a guide and bind the Cas9 protein to its complementary DNA strand. Cas9 protein would cleave the DNA strand at the binding site. dCas9 is a mutant of Cas9 that lacks DNase activity. dCas9 preserves the ability to bind gRNA and then to gRNA’s complementary DNA strand, but does not cut DNA double strands. Therefore dCas9/gRNA complex is able to inhibit expression without damaging DNA.
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dCas9 is the non-enzymatic variant of Cas9 form the CRISPR system. When it binds with target DNA, it cannot cause double strand breaks, which is why its name is 'dead Cas9".
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===Usage and Biology===
 
===Usage and Biology===
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2558003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2558003 SequenceAndFeatures</partinfo>
  

Revision as of 14:12, 17 October 2018


dCas9

Cas9 is the main protein of the CRISPR/Cas system II of Streptococcus pyogenes. CRISPR systems protect bacteria and archaea from phages by recognizing and cleaving of invading phage DNA. In practice, a short gRNA is used as a guide and bind the Cas9 protein to its complementary DNA strand. Cas9 protein would cleave the DNA strand at the binding site. dCas9 is a mutant of Cas9 that lacks DNase activity. dCas9 preserves the ability to bind gRNA and then to gRNA’s complementary DNA strand, but does not cut DNA double strands. Therefore dCas9/gRNA complex is able to inhibit expression without damaging DNA.