Difference between revisions of "Part:BBa K2622031"

 
 
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<partinfo>BBa_K2622031 short</partinfo>
 
<partinfo>BBa_K2622031 short</partinfo>
  
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Mstx-Lpp-OmpA-scFv_antiVGY long description
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Mstx-Lpp-OmpA-ScFv-antiVGY has a ScFv antibody against vaginoslysin (BBa_K2622004) attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the cholesterol-free membrane by erythrocyte membrane lysis assay. On the N-terminus it has a fused Mstx protein (BBa_K2622006) that improves recombinant membrane protein integration. There is no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase, since it has T7 promoter and terminator.
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This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:55, 17 October 2018


Mstx-Lpp-OmpA-scFv_antiVGY

Mstx-Lpp-OmpA-ScFv-antiVGY has a ScFv antibody against vaginoslysin (BBa_K2622004) attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the cholesterol-free membrane by erythrocyte membrane lysis assay. On the N-terminus it has a fused Mstx protein (BBa_K2622006) that improves recombinant membrane protein integration. There is no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase, since it has T7 promoter and terminator.

This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 792
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal NgoMIV site found at 747
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 845
    Illegal BsaI.rc site found at 20