Difference between revisions of "Part:BBa K2595008"

Revision as of 13:55, 17 October 2018

OmrB

OmrB is a small RNA that takes part in regulating the outer membrane protein composition. Research has shown OmrB to negatively regulate the expression of membrane proteins such as ompT and cirA. The effect of OmrB sRNA on ompT and cirA expression depends upon RNAse E enzyme and involves direct base pairing at the 5’ end of OmrB. The action of OmrB is also Hfq dependant. It is the 5’ end of OmrB that is involved in the sRNA’s regulatory function. A deletion mutant would result in a change in outer membrane protein composition, whilst overexpression of OmrB causes downregulation of CsgD expression. This results in inhibiting the formation of bacterial curli.

Usage and Biology

This synthetic version of OmrB follows the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, OmrB can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2595008 short</partinfo>
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+OmrB is a small RNA that takes part in regulating the outer membrane protein composition. Research has shown OmrB to negatively regulate the expression of membrane proteins such as ompT and cirA.
+The effect of OmrB sRNA on ompT and cirA expression depends upon RNAse E enzyme and involves direct base pairing at the 5’ end of OmrB. The action of OmrB is also Hfq dependant.
+It is the 5’ end of OmrB that is involved in the sRNA’s regulatory function. A deletion mutant would result in a change in outer membrane protein composition, whilst overexpression of OmrB causes downregulation of CsgD expression. This results in inhibiting the formation of bacterial curli.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This synthetic version of OmrB follows the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, OmrB can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence.  Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.
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