Difference between revisions of "Part:BBa K2684005"
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Revision as of 13:35, 17 October 2018


SpyCatcher-CotA

SpyCatcher fused with CotA by 2xGGGGS linker

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 841


We added the SpyCatcher sequence after cotA gene so that we can produce laccase protein with spycatcher (BBa_K2684005).