Difference between revisions of "Part:BBa K2622027"

Latest revision as of 13:28, 17 October 2018

Mstx-Lpp-OmpA-His

Mstx-Lpp-OmpA-His has a 6x His-tag attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the membrane. On the N-terminus it has a Mstx protein (BBa_K2622006) that improves recombinant membrane protein integration. There is no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.

This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 39
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 792
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 39
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 39
Illegal NgoMIV site found at 747
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 20

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2622027 short</partinfo>
-Mstx-Lpp-OmpA-His long description
+Mstx-Lpp-OmpA-His has a 6x His-tag attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the membrane. On the N-terminus it has a Mstx protein (BBa_K2622006) that improves recombinant membrane protein integration. There is no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.
+This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided.
 <!-- Add more about the biology of this part here