Difference between revisions of "Part:BBa K2719012:Design"

 
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===Design Notes===
 
===Design Notes===
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TCD5 coding device with blue chromoprotein  
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For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with <i>E. coli</i> BL21 (DE3) and with pSB1C3 plasmid. (Figure 1)
  
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"https://static.igem.org/mediawiki/2018/9/95/T--TecCEM--BBa_K2719010Diagram.png"
  
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<p><i>Figure 1.</i> Diagram of BBa_K2719012, leptin expression device.</p>
  
 
===Source===
 
===Source===

Revision as of 13:13, 17 October 2018


TCD5 coding device with blue chromoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1257


Design Notes

For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with E. coli BL21 (DE3) and with pSB1C3 plasmid. (Figure 1)

"T--TecCEM--BBa_K2719010Diagram.png"

Figure 1. Diagram of BBa_K2719012, leptin expression device.

Source

TCD5 coding device with blue chromoprotein

References