Difference between revisions of "Part:BBa K2719009:Design"

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===Design Notes===
 
===Design Notes===
 
This part is composed by a T7-lac promoter (BBa_R0184), a RBS binding site, a leptin coding region and a double terminator. Also, it was optimized for being cloned in pSB1C3 and transformed in <i>E. coli</i> BL21 (DE3).  This construct allows for an IPTG controlled expression of leptin.
 
This part is composed by a T7-lac promoter (BBa_R0184), a RBS binding site, a leptin coding region and a double terminator. Also, it was optimized for being cloned in pSB1C3 and transformed in <i>E. coli</i> BL21 (DE3).  This construct allows for an IPTG controlled expression of leptin.
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"https://static.igem.org/mediawiki/2018/c/c2/T--TecCEM--BBa_K2719009Diagram.png"
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<p><i>Figure 1.</i> Diagram of BBa_K2719009, leptin expression device.</p>
  
 
===Source===
 
===Source===

Latest revision as of 13:05, 17 October 2018


Leptin Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed by a T7-lac promoter (BBa_R0184), a RBS binding site, a leptin coding region and a double terminator. Also, it was optimized for being cloned in pSB1C3 and transformed in E. coli BL21 (DE3). This construct allows for an IPTG controlled expression of leptin.

"T--TecCEM--BBa_K2719009Diagram.png"

Figure 1. Diagram of BBa_K2719009, leptin expression device.

Source

Synthetic part.

References