Difference between revisions of "Part:BBa K2719007:Design"
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<partinfo>BBa_K2719007 short</partinfo> | <partinfo>BBa_K2719007 short</partinfo> | ||
| + | <p>This part is composed by the following subparts: Two promoters, one for RFP reporter cassette and the other one for collagen (with IPTG inducible T7 promoter [<partinfo>BBa_R0184</partinfo>]). Also, it has a double terminator in both genes. A RFP and collagen V-like coding regions are on it too. This part is designed for an IPTG regulated collagen expression and it has a RFP reporter to simplify comoly selection. </p> | ||
| + | "https://static.igem.org/mediawiki/2018/2/2d/T--TecCEM--BBa_K2719007Diagram.png" | ||
| + | <p><i>Figure 1.</i> Diagram of BBa_K2719007, collagen V-like expression device</p> | ||
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<partinfo>BBa_K2719007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2719007 SequenceAndFeatures</partinfo> | ||
Latest revision as of 12:57, 17 October 2018
Collagen V - Like Expression Device.
This part is composed by the following subparts: Two promoters, one for RFP reporter cassette and the other one for collagen (with IPTG inducible T7 promoter [BBa_R0184]). Also, it has a double terminator in both genes. A RFP and collagen V-like coding regions are on it too. This part is designed for an IPTG regulated collagen expression and it has a RFP reporter to simplify comoly selection.
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Figure 1. Diagram of BBa_K2719007, collagen V-like expression device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1046
Design Notes
Optimized for E.coli Rossetta (DE3).
Source
Synthetic part.