Difference between revisions of "Part:BBa K2719007"

Latest revision as of 12:52, 17 October 2018

Collagen V - Like Expression Device.

Collagen is optimized for Escherichia coli Rosetta 2-DE3 because it was the only strain modified for the detection of non-canonical amino acids. In this case, collagen has a non-canonical amino acid, specifically Hydroxyproline, in order to obtain a correctly binding between collagen and heparin. This protein was also cloned in pSB1C3 and in it, additionally, was cloned a Red Fluorescent Protein (RFP) to prove that the expression of collagen is taking place in the bacteria. This part contain a T7-lac promoter (BBa_R0184), a RBS (BBa_J611000) and a double TAATAA terminator (BBa_B0015). The prefix and suffix of every part were based on BioBrick RFC10.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1046

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2719007 short</partinfo>
-Collagen V - Like expression device with red flourescent protein (RFP) as reporter.  Optimized for use with E.coli
+Collagen is optimized for Escherichia coli Rosetta 2-DE3 because it was the only strain modified for the detection of non-canonical amino acids. In this case, collagen has a non-canonical amino acid, specifically Hydroxyproline, in order to obtain a correctly binding between collagen and heparin. This protein was also cloned in pSB1C3 and in it, additionally, was cloned a Red Fluorescent Protein (RFP) to prove that the expression of collagen is taking place in the bacteria. This part contain a T7-lac promoter (BBa_R0184), a RBS (BBa_J611000) and a double TAATAA terminator (BBa_B0015). The prefix and suffix of every part were based on BioBrick RFC10.
 <!-- Add more about the biology of this part here