Difference between revisions of "Part:BBa K2818002"
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<partinfo>BBa_K2818002 short</partinfo>
 
<partinfo>BBa_K2818002 short</partinfo>
  
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dPspCas13b-ADAR2DD(E488Q) is a fusion protein that catalyses the hydrolytic deamination of adenosine to form inosine in RNA molecules when used in conjunction with a guide RNA. Although there are preferred motifs, no Protospacer Adjacent Motif (PAM) is required.
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dPspCas13b-ADAR2DD(E488Q) is a fusion protein that catalyzes the hydrolytic deamination of adenosine to form inosine in RNA molecules when used in conjunction with a guide RNA. Although there are preferred motifs, no Protospacer Adjacent Motif (PAM) is required.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Two major domain is present in the construct
 
  
 
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Revision as of 12:50, 17 October 2018


Cas13b-NES-ADAR

dPspCas13b-ADAR2DD(E488Q) is a fusion protein that catalyzes the hydrolytic deamination of adenosine to form inosine in RNA molecules when used in conjunction with a guide RNA. Although there are preferred motifs, no Protospacer Adjacent Motif (PAM) is required.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1787
    Illegal BamHI site found at 839
    Illegal BamHI site found at 3310
    Illegal XhoI site found at 4011
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1520
    Illegal AgeI site found at 2396
  • 1000
    COMPATIBLE WITH RFC[1000]