Difference between revisions of "Part:BBa K2686005:Experience"
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| − | The part was designed and constructed in | + | The part was designed and constructed in the original pET14 vector from <bbpart>K2686001</bbpart>. The sequence was confirmed using Sanger sequencing. |
Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI. | Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI. | ||
Latest revision as of 12:50, 17 October 2018
The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI.
Applications of BBa_K2686005
User Reviews
UNIQ7989d1ffe9fd7eea-partinfo-00000001-QINU UNIQ7989d1ffe9fd7eea-partinfo-00000002-QINU