Difference between revisions of "Part:BBa K2719005:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Optimized for E.coli BL21.
 
  
  
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For the design if this part the following subparts: an IPTG inducible T7 promoter (BBa_R0184), in order to maximize the recombinant protein production.  An Anderson RBS (BBa_J61100), the Tenasin 5 Domain V+GST fusion protein coding site and a double terminator.  This part is designed for the IPTG regulated expression of the protein in E.coli BL21 (DE3), since this strain contains an IPTG inducible RNA polymerase T7.
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"https://static.igem.org/mediawiki/2018/8/89/T--TecCEM--BBa_K2719005Diagram.png"
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<p><i>Figure 1.</i> Diagram of BBa_K2719005, tenascin 5 domain V expression device.</p>
  
 
===Source===
 
===Source===

Revision as of 12:47, 17 October 2018


Tenascin Domain V Expression Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 416


Design Notes

For the design if this part the following subparts: an IPTG inducible T7 promoter (BBa_R0184), in order to maximize the recombinant protein production. An Anderson RBS (BBa_J61100), the Tenasin 5 Domain V+GST fusion protein coding site and a double terminator. This part is designed for the IPTG regulated expression of the protein in E.coli BL21 (DE3), since this strain contains an IPTG inducible RNA polymerase T7.

"T--TecCEM--BBa_K2719005Diagram.png"

Figure 1. Diagram of BBa_K2719005, tenascin 5 domain V expression device.

Source

Synthetic Part

References