Difference between revisions of "Part:BBa K2622006"
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| − | Mistic (Mstx) is an unique hydrophilic protein from Bacillus subtilis that works as a fusion tag and helps to integrate other proteins into lipid membranes more easily. When fused to the N-terminus of integral membrane proteins, MstX enables the cargo proteins to fold into their native conformations in the membrane, thus yielding a high-level expression. | + | Mistic (Mstx) is an unique hydrophilic protein from Bacillus subtilis that works as a fusion tag and helps to integrate other proteins into lipid membranes more easily. When fused to the N-terminus of integral membrane proteins, MstX enables the cargo proteins to fold into their native conformations in the membrane, thus yielding a high-level expression. Therefore, this biobrick has RFC12 prefix and suffix that allows to fuse proteins to the N or C-terminus in a stop codon free way. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:44, 17 October 2018
Mistic (Mstx)
Mistic (Mstx) is an unique hydrophilic protein from Bacillus subtilis that works as a fusion tag and helps to integrate other proteins into lipid membranes more easily. When fused to the N-terminus of integral membrane proteins, MstX enables the cargo proteins to fold into their native conformations in the membrane, thus yielding a high-level expression. Therefore, this biobrick has RFC12 prefix and suffix that allows to fuse proteins to the N or C-terminus in a stop codon free way.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]