Difference between revisions of "Part:BBa K2622005"
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A disulphide bond - free GFP nanobody that binds to GFP (Kd(nM)=0.13). Its expression levels in E. coli are recognized to be very low - 20µg/L of E. coli culture. | A disulphide bond - free GFP nanobody that binds to GFP (Kd(nM)=0.13). Its expression levels in E. coli are recognized to be very low - 20µg/L of E. coli culture. | ||
This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes. | This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 12:28, 17 October 2018
GFP Nanobody
A disulphide bond - free GFP nanobody that binds to GFP (Kd(nM)=0.13). Its expression levels in E. coli are recognized to be very low - 20µg/L of E. coli culture. This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]