Difference between revisions of "Part:BBa K2622004"
Line 5: Line 5:
 
Single chain antibody against vaginolysin. It has heavy chain connected to a heavy chain via gly/ser rich linker. This single chain binds to the vaginolysin and inhibts its lysation of cholesterol rich membranes. It could be used in erythrocyte lysis assay.  
 
Single chain antibody against vaginolysin. It has heavy chain connected to a heavy chain via gly/ser rich linker. This single chain binds to the vaginolysin and inhibts its lysation of cholesterol rich membranes. It could be used in erythrocyte lysis assay.  
  
−
This biobrick has RFC10 prefix and suffix and  
+
This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:18, 17 October 2018


scFv_antiVGY

Single chain antibody against vaginolysin. It has heavy chain connected to a heavy chain via gly/ser rich linker. This single chain binds to the vaginolysin and inhibts its lysation of cholesterol rich membranes. It could be used in erythrocyte lysis assay.

This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41