Difference between revisions of "Part:BBa K2719000"
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Glutathione S-transferase (GST) is a peptide derived from Schistosoma japonicum, in which a target protein may be fused on the N-terminal. This fusion allows the purification of the target protein using glutathione affinity and maximize the presence of the protein in soluble state, rather than on inclusion bodies. In addition, this fusion protein gives stability to the domain of Tenascin V and for this is necessary to use a polyproline linker to maintain the original three dimensional structure of the protein. This tag may be later removed by adding a protease recognition site between GST and the target protein (Harper & Speicher, 2013). | Glutathione S-transferase (GST) is a peptide derived from Schistosoma japonicum, in which a target protein may be fused on the N-terminal. This fusion allows the purification of the target protein using glutathione affinity and maximize the presence of the protein in soluble state, rather than on inclusion bodies. In addition, this fusion protein gives stability to the domain of Tenascin V and for this is necessary to use a polyproline linker to maintain the original three dimensional structure of the protein. This tag may be later removed by adding a protease recognition site between GST and the target protein (Harper & Speicher, 2013). | ||
Revision as of 10:55, 17 October 2018
GST
Glutathione S-transferase (GST) is a peptide derived from Schistosoma japonicum, in which a target protein may be fused on the N-terminal. This fusion allows the purification of the target protein using glutathione affinity and maximize the presence of the protein in soluble state, rather than on inclusion bodies. In addition, this fusion protein gives stability to the domain of Tenascin V and for this is necessary to use a polyproline linker to maintain the original three dimensional structure of the protein. This tag may be later removed by adding a protease recognition site between GST and the target protein (Harper & Speicher, 2013).
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85