Difference between revisions of "Part:BBa K2833014"
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<p>Previous research has found that expressing <i>Vitreoscilla</i> hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.<sup>[1]</sup> Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.<sup>[2]</sup> Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.</p> | <p>Previous research has found that expressing <i>Vitreoscilla</i> hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.<sup>[1]</sup> Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.<sup>[2]</sup> Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.</p> | ||
− | <p>For surface display of VHb, we constructed this part to fuse the surface display protein INP(from < | + | <p>For surface display of VHb, we constructed this part to fuse the surface display protein INP(from <html><a href='https://parts.igem.org/Part:BBa_K523013'>BBa_K523013</a></html>) to the N-terminal of the VHb protein.(figure 1) |
+ | [[File:T--BJRS China--014.jpg|thumbnail|center|800px|<b>Figure 1. The framework of BBa_K2833014</b>]]<br><br> | ||
===References=== | ===References=== | ||
<p>1.Lei Zhang <i>et al.</i>. Recent developments and future prospects of <i>Vitreoscilla</i> hemoglobin application in metabolic engineering. <i>Biotechnology Advances</i> <b>25</b>(2007).</font></p> | <p>1.Lei Zhang <i>et al.</i>. Recent developments and future prospects of <i>Vitreoscilla</i> hemoglobin application in metabolic engineering. <i>Biotechnology Advances</i> <b>25</b>(2007).</font></p> |
Revision as of 10:38, 17 October 2018
J23104+B0032+INP+VHb+Ter
Previous research has found that expressing Vitreoscilla hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.[1] Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.[2] Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.
For surface display of VHb, we constructed this part to fuse the surface display protein INP(from BBa_K523013) to the N-terminal of the VHb protein.(figure 1)
References
<p>1.Lei Zhang et al.. Recent developments and future prospects of Vitreoscilla hemoglobin application in metabolic engineering. Biotechnology Advances 25(2007).</font>2.Pengfei Ouyang et al.. Increasing oxygen availability for improving poly(3-hydroxybutyrate) production by Halomonas. Metabolic Engineering 45(2018).</font>
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 467
- 1000COMPATIBLE WITH RFC[1000]