Difference between revisions of "Part:BBa K2833014"

Revision as of 10:35, 17 October 2018

J23104+B0032+INP+VHb+Ter

Previous research has found that expressing Vitreoscilla hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.^[1] Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.^[2] Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.

For surface display of VHb, we constructed this part to fuse the surface display protein INP(from <http><a href='https://parts.igem.org/Part:BBa_K523013'>BBa_K523013</a></http>) to the N-terminal of the VHb protein.(figure 1)

References

<p>1.Lei Zhang et al.. Recent developments and future prospects of Vitreoscilla hemoglobin application in metabolic engineering. Biotechnology Advances 25(2007).</font>

2.Pengfei Ouyang et al.. Increasing oxygen availability for improving poly(3-hydroxybutyrate) production by Halomonas. Metabolic Engineering 45(2018).</font>

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 467
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2833014 short</partinfo>
-Previous research has found that expressing <i>Vitreoscilla</i> hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.<sup>[1]</sup> Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.<sup>[2]</sup> Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.</font></p>
+<p>Previous research has found that expressing <i>Vitreoscilla</i> hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.<sup>[1]</sup> Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.<sup>[2]</sup> Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.</p>
+<p>For surface display of VHb, we constructed this part to fuse the surface display protein INP(from <http><a href='https://parts.igem.org/Part:BBa_K523013'>BBa_K523013</a></http>) to the N-terminal of the VHb protein.(figure 1)
 ===References===
 <p>1.Lei Zhang <i>et al.</i>. Recent developments and future prospects of <i>Vitreoscilla</i> hemoglobin application in metabolic engineering. <i>Biotechnology Advances</i> <b>25</b>(2007).</font></p>
 <p>2.Pengfei Ouyang <i>et al.</i>. Increasing oxygen availability for improving poly(3-hydroxybutyrate) production by <i>Halomonas</i>. <i>Metabolic Engineering</i> <b>45</b>(2018).</font></p>
-==Sequence and Features==
 <!-- Add more about the biology of this part here