Difference between revisions of "Part:BBa K091150:Design"

 
 
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===Design Notes===
 
===Design Notes===
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pLas' Testing Construct C has the coding region for luxR preceding the region for LasR, but the order of these regions is reversed in pLas' Testing Construct D.  
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pLas' Testing Construct C has the coding region for LasR preceding the region for LuxR, but the order of these regions is reversed in pLas' Testing Construct D.  
  
  

Latest revision as of 19:01, 18 July 2008

pLas' Testing Construct C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2591


Design Notes

pLas' Testing Construct C has the coding region for LasR preceding the region for LuxR, but the order of these regions is reversed in pLas' Testing Construct D.


Source

The sources of the quorum sensing components are the modified lasI regulatory region in P. aeruginosa and the lux components from V. fisheri.

References