Difference between revisions of "Part:BBa K2789023"
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===Application=== | ===Application=== | ||
This part can be added before a coding sequence to express it with the PPX. | This part can be added before a coding sequence to express it with the PPX. | ||
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+ | <div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/2/2b/T--WHU-China--parts-11.png" style="width:800px;"> | ||
+ | </div> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 08:49, 17 October 2018
PPX+RBS
This part actually is a by-product during PCR but we found that this part can make a contribution to pathway establishment! the coding sequence PPX is originally from 15_York, and both two teams(15_York and 18_WHU) made a good verification of its function. Since it has a RBS just after its cds, you could build a pathway by just adding another coding sequence downstream this sequence.
We designed a primer to copy the PPX+RBS on the BBa_K2789032.
Application
This part can be added before a coding sequence to express it with the PPX.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 872