Difference between revisions of "Part:BBa K2549032"
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===Biology===
 
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Different transcription factors interaction with multiple binding sites
 
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Promotor operator numbers can have an impact on tuning output
 
  
 
=====Synthetic promotor operators regulated by artificial zinc finger-based transcription factors=====
 
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[[File:ZF3.jpg|none|240px|thumb|Khalil AS et al stated:''Tuning up output strength by increasing ZF operator number in synthetic promoter (sTF43-8).'']]
 
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[[File:model.png|none|480px|thumb|'''Flow cytometry results of different transcription factors interaction with multiple binding sites.''']]
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Promotor operator numbers can have an impact on tuning output
  
 
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Revision as of 08:29, 17 October 2018

4*ZF21.16-minCMV-2*ZF43.8

This part is one of the response elements of our amplifier, also executing the combiner function. 4*ZF21.16 binding sites (Part:BBa_K2446008) and 2*ZF43.8 binding sites (Part:BBa_K2446059) can bind to different zinc finger-based transcription activator ZF21.16-VP64 (Part:BBa_K2549023) and zinc finger-based repressor ZF43.8-KRAB (Part:BBa_K2446041), respectively, with high orthogonality. Minimal CMV (Part:BBa_K2549049) is a promotor providing very low basal expression and high maximal expression after induction. This part was designed to construct our NIMPLY logic gate and test our multiple binding sites amplifier model[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Biology

Synthetic promotor operators regulated by artificial zinc finger-based transcription factors

Khalil AS et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality[2].

Khalil AS et al stated:sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.
Khalil AS et al stated:Tuning up output strength by increasing ZF operator number in synthetic promoter (sTF43-8).

Characterization

It works as we designed
Flow cytometry results of different transcription factors interaction with multiple binding sites.


Promotor operator numbers can have an impact on tuning output


References

  1. ↑ http://2018.igem.org/Team:Fudan/Model#Transcriptional_Amplifer
  2. ↑ A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58 PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045