Difference between revisions of "Part:BBa K2710002"
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===Usage and Biology===
 
===Usage and Biology===
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<p>Alpha prefoldin (aPFD) (15.7 kDa) and beta prefoldin (bPFD) (13.8 kDa) derived from <em>Methanobacterium thermoautotrophicum</em> oligomerise to form heterohexameric structures (87 kDa), consisting of 2 alpha subunits and 4 beta subunits<sup>2</sup>. The prefoldin hexamer is assembled through interactions between beta hairpins in each subunit. These beta hairpins form two 8 stranded up and down beta barrels.
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<p>Alpha prefoldin (aPFD) (15.7 kDa) and beta prefoldin (bPFD) (13.8 kDa) derived from the thermophilic arcahea <em>Methanobacterium thermoautotrophicum</em> oligomerise to form a heterohexameric structures (87 kDa), consisting of two alpha subunits and four beta subunits<sup>1</sup>. The prefoldin hexamer is assembled through interactions between beta hairpins in each subunit. These beta hairpins form two 8 stranded up and down beta barrels.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2710002 parameters</partinfo>
 
<partinfo>BBa_K2710002 parameters</partinfo>
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<h2>References<h2>
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<ol>
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<li>Siegert, R., Leroux, M. R., Scheufler, C., Hartl, F. U. & Moarefi, I. Structure of the molecular chaperone prefoldin: unique interaction of multiple coiled coil tentacles with unfolded proteins. Cell 103, 621–32 (2000).</li>
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</ol>
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</div>
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</div>

Revision as of 07:53, 17 October 2018


His-Alpha Prefoldin with SpyCatcher

6xHis Alpha Prefoldin with Spy-Catcher


Usage and Biology

Alpha prefoldin (aPFD) (15.7 kDa) and beta prefoldin (bPFD) (13.8 kDa) derived from the thermophilic arcahea Methanobacterium thermoautotrophicum oligomerise to form a heterohexameric structures (87 kDa), consisting of two alpha subunits and four beta subunits1. The prefoldin hexamer is assembled through interactions between beta hairpins in each subunit. These beta hairpins form two 8 stranded up and down beta barrels. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]