Difference between revisions of "Part:BBa I719005"
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Summary: We adapted the part to be able to assemble transcriptional units with the Golden Gate assembly method
 
Summary: We adapted the part to be able to assemble transcriptional units with the Golden Gate assembly method
 
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Documentation:
 
 
===Contribution===
 
===Contribution===
 
Group: Valencia_UPV iGEM 2018
 
Group: Valencia_UPV iGEM 2018
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Documentation:
 
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<html><p>In order to create our complete <a href=”2018.igem.org/Team:Valencia_UPV/Part_Collection”> part collection </a> of parts compatible with the Golden Gate assembly method, we made the part <a href=”parts.igem.org/Part:BBa_K2656000”> BBa_K2656000</a> which is this part adapted to the Golden Gate technology.</p></html>
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In order to create our complete [http://2018.igem.org/Team:Valencia_UPV/Part_Collection part collection] of parts compatible with the Golden Gate assembly method, we made the part [https://parts.igem.org/Part:BBa_K2656000 BBa_K2656000] which is this part adapted to the Golden Gate technology.

Revision as of 07:47, 17 October 2018

T7 Promoter

Constitutive promoter derived from the T7 bacteriophage. Allows high expression of proteins only when the T7 polymerase is present. This part is identical to the part BBa_R0085 which currently hasn't been built.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization in vitro

This part has been characterized for temperature sensitivity in the Cell-Free Chassis by iGEM Imperial 2007. For more detail, please check the testing [http://2007.igem.org/Imperial/Wet_Lab/Protocols/Prot1.9 protocols]and [http://2007.igem.org/Imperial/Wet_Lab/Results/Res1.9 results].

I719005 invitro.png
Parameter Value and Description
Optimum temperature The T7 promoter has a highest output at 25°C, with a one-fold increase in GFP molecules synthesized compared to 37°C. The T7 promoter also has a minimal amount of output at 4°C.
Expression Life-span The rate of GFP synthesis by the T7 promoter reaches a peak at around 30-60 minutes.
Peak Expression The production of GFP decreases to minimal levels after 2 hours and tends towards nil after 4 hours.

Team Warsaw 2010's measurement

Absolute promoter strength: 41,8pg RNA/minute/ug substrate DNA. It equals 8,92 microPoPS

Contribution

Group: Valencia_UPV iGEM 2018
Author: Adrián Requena Gutiérrez, Carolina Ropero
Summary: We adapted the part to be able to assemble transcriptional units with the Golden Gate assembly method

Contribution

Group: Valencia_UPV iGEM 2018
Author: Adrián Requena Gutiérrez, Carolina Ropero
Summary: We adapted the part to be able to assemble transcriptional units with the Golden Gate assembly method
Documentation: In order to create our complete [http://2018.igem.org/Team:Valencia_UPV/Part_Collection part collection] of parts compatible with the Golden Gate assembly method, we made the part BBa_K2656000 which is this part adapted to the Golden Gate technology.