Difference between revisions of "Part:BBa K2588022"
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<html> <p>This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 unsaturated fatty acid (myristic acid) as seen in fig. 1.<sup>1</sup> The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occurs as an α<sub>2</sub>β<sub>2</sub> heterotetramer.<sup>2,3</sup> | <html> <p>This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 unsaturated fatty acid (myristic acid) as seen in fig. 1.<sup>1</sup> The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occurs as an α<sub>2</sub>β<sub>2</sub> heterotetramer.<sup>2,3</sup> | ||
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− | <img src="https://static.igem.org/mediawiki/parts/b/b3/Fattyacidssynthesisecoli.png"> | + | <img src="https://static.igem.org/mediawiki/parts/b/b3/Fattyacidssynthesisecoli.png" style="width: 100%"> |
<p>Fig. 1 (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.<sup>1</sup> | <p>Fig. 1 (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.<sup>1</sup> | ||
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Revision as of 07:23, 17 October 2018
myristic acid synthesis module, glucose-induced
This module consists of the 5 genes encoding the 4 subunits of the actetyl-coenzyme A carboxylase complex and the thioesterase 1 from the e. coli genome. These proteins play a vital role in the fatty acid bio-synthesis in e. coli and lead to a significantly higher production of the C-14 unsaturated fatty acid (myristic acid) as seen in fig. 1.1 The ribosome binding sites from the registry and their respective strengths used in this assembly were chosen in order to account for the fact that accB and accC are transcribed as a single mRNA and accA and accD encode for subunits alpha and beta of the acetyl-CoA carboxyltransferase, which occurs as an α2β2 heterotetramer.2,3
![](https://static.igem.org/mediawiki/parts/b/b3/Fattyacidssynthesisecoli.png)
Fig. 1 (a) fatty acid synthesis in e. coli. (b) fatty acid production. (c) HPLC of fatty acids phenacyl esters.1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 144
Illegal NheI site found at 167
Illegal NotI site found at 1841 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3807
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2317
Illegal AgeI site found at 1097
Illegal AgeI site found at 2851 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1035
Illegal SapI.rc site found at 1232