Difference between revisions of "Part:BBa K2862020"

 
 
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<partinfo>BBa_K2862020 short</partinfo>
 
<partinfo>BBa_K2862020 short</partinfo>
  
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pSB1C3_BASIC (iGEM vector with BASIC sites)
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This version of the pSB1C3 iGEM submission vector was developed by researchers at Imperial College to provide a general standardised storage vector that can be used for BioBrick, Golden Gate and BASIC assembly. The backbone contains an mScarlet fluorescent chromoprotein dropout cassette, to allow for simple negative selection of successful assemblies using this plasmid. The dropout is flanked by both the BASIC and BioBrick Prefix and Suffix sequences, meaning 3A and various BsaI based assembly methods can be used.
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The 2018 Imperial College iGEM team (pixcell) used this part for all their submissions into the iGEM registry. They submitted the part on the behalf of the researchers and did not design or construct it.
  
  

Latest revision as of 07:20, 17 October 2018


pSB1C3 for Multiple Assembly Standards (3A, BASIC and Golden Gate)

This version of the pSB1C3 iGEM submission vector was developed by researchers at Imperial College to provide a general standardised storage vector that can be used for BioBrick, Golden Gate and BASIC assembly. The backbone contains an mScarlet fluorescent chromoprotein dropout cassette, to allow for simple negative selection of successful assemblies using this plasmid. The dropout is flanked by both the BASIC and BioBrick Prefix and Suffix sequences, meaning 3A and various BsaI based assembly methods can be used.

The 2018 Imperial College iGEM team (pixcell) used this part for all their submissions into the iGEM registry. They submitted the part on the behalf of the researchers and did not design or construct it.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 75
    Illegal suffix found at 1068
    Illegal SpeI site found at 151
    Illegal SpeI site found at 171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 75
    Illegal NheI site found at 121
    Illegal NheI site found at 144
    Illegal SpeI site found at 151
    Illegal SpeI site found at 171
    Illegal SpeI site found at 1069
    Illegal PstI site found at 1083
    Illegal NotI site found at 81
    Illegal NotI site found at 1076
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 75
    Illegal BamHI site found at 873
    Illegal XhoI site found at 2100
    Illegal XhoI site found at 2992
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 75
    Illegal suffix found at 1069
    Illegal SpeI site found at 151
    Illegal SpeI site found at 171
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 75
    Plasmid lacks a suffix.
    Illegal XbaI site found at 90
    Illegal SpeI site found at 151
    Illegal SpeI site found at 171
    Illegal SpeI site found at 1069
    Illegal PstI site found at 1083
    Illegal AgeI site found at 734
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1054
    Illegal BsaI.rc site found at 104