Difference between revisions of "Part:BBa K2611004"

 
Line 3: Line 3:
 
<partinfo>BBa_K2611004 short</partinfo>
 
<partinfo>BBa_K2611004 short</partinfo>
  
−
We designed this sgRNA that are complementary to the CDS of J23100 constitutive promoter using a special algorithm. This sgRNA can direct dCas9 protein to combine with the J23100 constitutive promoter to block the transcription. As a result, the expression of the downstream will decrease.  
+
We designed this sgRNA that are complementary to the J23100 constitutive promoter using a special algorithm. This sgRNA can direct dCas9 protein to combine with the J23100 constitutive promoter to block the transcription. As a result, the expression of the downstream will decrease.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:19, 17 October 2018


sgRNA (J23100)

We designed this sgRNA that are complementary to the J23100 constitutive promoter using a special algorithm. This sgRNA can direct dCas9 protein to combine with the J23100 constitutive promoter to block the transcription. As a result, the expression of the downstream will decrease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]