Difference between revisions of "Part:BBa K2765026"
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Revision as of 07:19, 17 October 2018
GAL1p+yno1+CYC1t
This part consists of the promoter gal1, redox sensor yno1 and the terminator cyc1. The promoter gal1 is an constitutive promoter from Saccharomyces cerevisiae CENPK2-1C.yno1(yeast NADPH oxidase 1) is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion encodes a genuine NADPH oxidase (NOX enzymes),(see the details in part:BBA-K2765007).And thus the target gene yno1 is induced by galactose and repressed by glucose.as shown in Fig.1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2452
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 669
Illegal XhoI site found at 2430 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 286
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2281