Difference between revisions of "Part:BBa K091117"

 
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This promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region  modified from CATAAA to TATAAA to reflect our pLasXOR promoter design.  Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein.  
 
This promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region  modified from CATAAA to TATAAA to reflect our pLasXOR promoter design.  Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein.  
  
pLas' serves as a control for the evaluation of the pLasXOR promoter, part BBa_K091118.  
+
pLas' serves as a control for the evaluation of the pLas+ Lux- promoter, part BBa_K091146.  
  
 
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Latest revision as of 14:45, 17 July 2008

pLas promoter

This promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region modified from CATAAA to TATAAA to reflect our pLasXOR promoter design. Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein.

pLas' serves as a control for the evaluation of the pLas+ Lux- promoter, part BBa_K091146.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]