Difference between revisions of "Part:BBa K2611001"

Revision as of 06:26, 17 October 2018

spacer J23101-GFP

   We added a spacer sequence and NGG site closely before the promoter (BBa_B0034). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of GFP will be repressed.

To be used in pSB1C3.

   We selected part J23101-GFP (BBa_J364000) as a reporter to test the function of the modified promoter and measure the repression level. The modified promoter does not influence the function of GFP gene as the GFP fluorescence intensity of the modified part is even stronger than the original one (Figure 3).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 30
Illegal NheI site found at 53
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 728

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2611001 short</partinfo>
-We added a spacer sequence and NGG site closely before the promoter (BBa_B0034). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of GFP will be repressed.
+    We added a spacer sequence and NGG site closely before the promoter (BBa_B0034). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of GFP will be repressed.
 To be used in pSB1C3.
+    We selected part J23101-GFP (BBa_J364000) as a reporter to test the function of the modified promoter and measure the repression level. The modified promoter does not influence the function of GFP gene as the GFP fluorescence intensity of the modified part is even stronger than the original one (Figure 3).
 [[File:SCU_China-2018_spacer_J23101_GFP.png]]