Difference between revisions of "Part:BBa K2611000"

 
 
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<partinfo>BBa_K2611000 short</partinfo>
 
<partinfo>BBa_K2611000 short</partinfo>
  
This sgRNA targets part "spacer J23101-GFP"(BBa_K2611001), and can combines with it's spacer sequence. This sgRNA here can combine with dCas9 protein to direct the complex to the upstream sequence of promoter of GFP to prevent transcription initiation. So, we suggest to use both this part and the GFP we have already modified (BBa_K261101).  
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This sgRNA targets part "spacer J23101-GFP"(BBa_K2611001), and can combines with it's spacer sequence. This sgRNA here can combine with dCas9 protein to direct the complex to the upstream sequence of promoter of GFP to prevent transcription initiation. So, we suggest to use both this part and the GFP we have already modified (BBa_K2611001).  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:18, 17 October 2018


sgRNA (spacer J23101-GFP)

This sgRNA targets part "spacer J23101-GFP"(BBa_K2611001), and can combines with it's spacer sequence. This sgRNA here can combine with dCas9 protein to direct the complex to the upstream sequence of promoter of GFP to prevent transcription initiation. So, we suggest to use both this part and the GFP we have already modified (BBa_K2611001).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]