Difference between revisions of "Part:BBa K2705006:Design"
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===Design Notes===
 
===Design Notes===
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It was applied to sieve Bacillus amyloliquefaciens LL3 bacteria with high level glutamate under tetracycline circumstance, which can produce more products whose precursor is glutamate such as γ-PGA. Since LL3 can produce glutamate by itself, high-producing cells can survive based on the amount of precursor.
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It was applied to sieve <i>Bacillus amyloliquefaciens</i> LL3 bacteria with high level glutamate under tetracycline circumstance, which can produce more products whose precursor is glutamate such as &#947;-PGA. Since LL3 can produce glutamate by itself, high-producing cells can survive based on the amount of precursor.
  
  

Revision as of 06:15, 17 October 2018


PgltAB-LacI-Pgrac-TetA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1229
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was applied to sieve Bacillus amyloliquefaciens LL3 bacteria with high level glutamate under tetracycline circumstance, which can produce more products whose precursor is glutamate such as γ-PGA. Since LL3 can produce glutamate by itself, high-producing cells can survive based on the amount of precursor.


Source

PgltAB is acqcuired from LL3 by PCR, LacI is from vector pTH01 by PCR, Pgrac is from vector pTH01 by PCR, TetA is from vector pWH1520 by PCR.

References