Difference between revisions of "Part:BBa J72009"
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− | This plasmid, referred to in-house as pBca1101 is available in Genbank/ApE format [[Image:JCA_pBca1101.txt here]]. The plasmid is comparable to pBca9145, but it inserts an RFP open reading frame into the BamHI and XhoI sites. | + | This plasmid, referred to in-house as pBca1101 is available in Genbank/ApE format [[Image:JCA_pBca1101.txt | here]]. The plasmid is comparable to pBca9145, but it inserts an RFP open reading frame into the BamHI and XhoI sites. |
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Latest revision as of 22:06, 16 July 2008
pBca1101 RFP expression plasmid in BBb
This plasmid, referred to in-house as pBca1101 is available in Genbank/ApE format File:JCA pBca1101.txt. The plasmid is comparable to pBca9145, but it inserts an RFP open reading frame into the BamHI and XhoI sites.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 885 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 885 - 21INCOMPATIBLE WITH RFC[21]Illegal prefix found in sequence at 1
Illegal BamHI site found at 29
Illegal XhoI site found at 879 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 885 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 885
Illegal AgeI site found at 589
Illegal AgeI site found at 701 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1972