Difference between revisions of "Part:BBa K2753012"

 
Line 3: Line 3:
 
<partinfo>BBa_K2753012 short</partinfo>
 
<partinfo>BBa_K2753012 short</partinfo>
  
−
This part can synthesize trans-cis iridoid. We use this part to knock in to yeast and converts 8-oxogeraniol to cis-trans iridodial and cis-trans nepetalactol.
+
Catharanthus roseus ridoid synthase (ISY) codon-optimised for use in S. cerevisiae catalysing the reductive cyclisation to produce nepetalactol. Expression of this circuit is regulated by a yeast constitutive promoter pRPL18B and terminator tTDH1.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:39, 17 October 2018


pSB1C3-pRPL18P promoter-CrISY-tTDH1

Catharanthus roseus ridoid synthase (ISY) codon-optimised for use in S. cerevisiae catalysing the reductive cyclisation to produce nepetalactol. Expression of this circuit is regulated by a yeast constitutive promoter pRPL18B and terminator tTDH1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 701
    Illegal BglII site found at 728
    Illegal XhoI site found at 1884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]