Difference between revisions of "Part:BBa K2753011"

 
Line 3: Line 3:
 
<partinfo>BBa_K2753011 short</partinfo>
 
<partinfo>BBa_K2753011 short</partinfo>
  
−
We use this part to knock in to yeast and converts 8-hydroxygeraniol to 8-oxogeraniol.  
+
Catharanthus roseus geraniol oxidoreductase (GOR) codon-optimised for use in S. cerevisiae, responsible for oxidation of 8-hydroxygeraniol to 8-oxogeranial. Expression of this circuit is regulated by a yeast constitutive promoter pTEF1 and terminator tENO1.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:39, 17 October 2018


pSB1C3-TEF1 promoter-GOR-tENO1

Catharanthus roseus geraniol oxidoreductase (GOR) codon-optimised for use in S. cerevisiae, responsible for oxidation of 8-hydroxygeraniol to 8-oxogeranial. Expression of this circuit is regulated by a yeast constitutive promoter pTEF1 and terminator tENO1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]