Difference between revisions of "Part:BBa K2753010"

 
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<partinfo>BBa_K2753010 short</partinfo>
 
<partinfo>BBa_K2753010 short</partinfo>
  
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The original use of the part is to convert geraniol to 8-hydroxygeraniol, so we knock it in to yeast and converts geraniol to 8-hydroxygeranial.
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The complete genetic circuit encoding for Catharanthus roseus 8-hydroxylase (G8H) codon-optimised for use in S. cerevisiae, responsible for the hydroxylation of geraniol by geraniol to form 8-hydroxygeraniol. Expression of this circuit is regulated by a strong yeast constitutive promoter pTDH3 and terminator tENO2.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:25, 17 October 2018


pSB1C3-TDH3 promoter-G8H-tENO2

The complete genetic circuit encoding for Catharanthus roseus 8-hydroxylase (G8H) codon-optimised for use in S. cerevisiae, responsible for the hydroxylation of geraniol by geraniol to form 8-hydroxygeraniol. Expression of this circuit is regulated by a strong yeast constitutive promoter pTDH3 and terminator tENO2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 956
    Illegal BglII site found at 1034
    Illegal BglII site found at 1442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]