Difference between revisions of "Part:BBa K2705005"
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<partinfo>BBa_K2705005 short</partinfo>
 
<partinfo>BBa_K2705005 short</partinfo>
  
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This part includes the promoter <i>gltA</i> and <i>gltB</i> and GFP, the expression of GFP is repressed by high level cellular glutamate because <i>gltA</i> and <i>gltB</i> is upregulated by GltC, which is repressed by cellular glutamate level. A restriction enzyme KpnI cutting site is between <i>gltA</i> and <i>gltB</i> and GFP.
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This part includes the promoter P<sub>''gltAB''</sub> and GFP, the expression of GFP is repressed by high level cellular glutamate because P<sub>''gltAB''</sub> is upregulated by GltC, which is repressed by cellular glutamate level. A restriction enzyme KpnI cutting site is between P<sub>''gltAB''</sub> and GFP.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 05:20, 17 October 2018


PgltAB-GFP

This part includes the promoter PgltAB and GFP, the expression of GFP is repressed by high level cellular glutamate because PgltAB is upregulated by GltC, which is repressed by cellular glutamate level. A restriction enzyme KpnI cutting site is between PgltAB and GFP. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 671
    Illegal XhoI site found at 572
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]