Difference between revisions of "Part:BBa J72008:Design"

Revision as of 21:14, 16 July 2008

phi80 integration helper plasmid pInt80-649

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 878
Illegal XbaI site found at 2279
Illegal SpeI site found at 939
Illegal SpeI site found at 4519
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 878
Illegal NheI site found at 884
Illegal SpeI site found at 939
Illegal SpeI site found at 4519
Illegal NotI site found at 4838
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 878
Illegal BglII site found at 2722
Illegal BglII site found at 3416
Illegal BamHI site found at 1263
Illegal XhoI site found at 1576
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 878
Illegal XbaI site found at 2279
Illegal SpeI site found at 939
Illegal SpeI site found at 4519
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 878
Illegal XbaI site found at 2279
Illegal SpeI site found at 939
Illegal SpeI site found at 4519
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 5484

Design Notes

N/A

Source

Constructed from CRIM integration plasmid pAH123.  See ACCESSION AY048726 and PMID: 11591683 for details.
PCR ca648F/R on pAH123		(~5200 bp, BglII/XbaI)
PCR ca649F/R on BW23474 gen	(1154 bp, BamHI/XbaI)
Ligate, product is pInt80-649	(6335 bp)

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J72008 short</partinfo>
@@ Line 12: / Line 11: @@
 ===Source===
+<pre>
-Constructed from CRIM integration plasmid pAH????.  See PMID #### for details.
+Constructed from CRIM integration plasmid pAH123.  See ACCESSION AY048726 and PMID: 11591683 for details.
+PCR ca648F/R on pAH123		(~5200 bp, BglII/XbaI)
+PCR ca649F/R on BW23474 gen	(1154 bp, BamHI/XbaI)
+Ligate, product is pInt80-649	(6335 bp)
+</pre>
 ===References===