Difference between revisions of "Part:BBa K2832015:Design"

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===Design Notes===
 
===Design Notes===
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Design considerations...
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https://static.igem.org/mediawiki/parts/4/44/T--PSB1C3-_apFAB82-mRFP1_map.png
  
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Plasmid map that includes BIOFAB promoter apFAB82
  
  

Revision as of 04:08, 17 October 2018


BIOFAB Modular Promoter apFAB82 Driven mRFP1 Generator ( ---> mRFP1 )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 630
    Illegal AgeI site found at 742
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T--PSB1C3-_apFAB82-mRFP1_map.png

Plasmid map that includes BIOFAB promoter apFAB82


Source

Source...

References

Mutalik,V. Guimaraes, J. Cambray, G. Lam, C. Christoffersen, M. Mai, Q. Tran, A Morgan Paull, Keasling, J. Arkin, A. Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements: Nature Methods, 354-360