Difference between revisions of "Part:BBa K2825002"
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Inducible promoter for protocatechuic acid (PCA). The PcaU transcription factor represses its own transcription as well as a target transcript when PCA is not present by binding to the operator region. When PCA is present, PcaU releases the region and transcription of the target transcript occurs.
 
Inducible promoter for protocatechuic acid (PCA). The PcaU transcription factor represses its own transcription as well as a target transcript when PCA is not present by binding to the operator region. When PCA is present, PcaU releases the region and transcription of the target transcript occurs.
  
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===Usage and Biology===
 
===Usage and Biology===
 
PcaU has been inserted into the pGLO plasmid, in which it expresses GFP when induced by PCA. Fluorescent intensity scales hyperbolically with PCA concentration. Above 10mM concentration, PCA was toxic to BL21 E. coli containing the PcaU reporter.
 
PcaU has been inserted into the pGLO plasmid, in which it expresses GFP when induced by PCA. Fluorescent intensity scales hyperbolically with PCA concentration. Above 10mM concentration, PCA was toxic to BL21 E. coli containing the PcaU reporter.
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<img src="https://static.igem.org/mediawiki/2018/a/a9/T--UMaryland--modelresults.png"
 
  
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<img src="https://static.igem.org/mediawiki/2018/a/a9/T--UMaryland--modelresults.png">
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2825002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2825002 SequenceAndFeatures</partinfo>

Revision as of 01:23, 17 October 2018


PcaU inducible promoter with high sensitivity

Inducible promoter for protocatechuic acid (PCA). The PcaU transcription factor represses its own transcription as well as a target transcript when PCA is not present by binding to the operator region. When PCA is present, PcaU releases the region and transcription of the target transcript occurs.


Usage and Biology

PcaU has been inserted into the pGLO plasmid, in which it expresses GFP when induced by PCA. Fluorescent intensity scales hyperbolically with PCA concentration. Above 10mM concentration, PCA was toxic to BL21 E. coli containing the PcaU reporter.

<img src="T--UMaryland--modelresults.png">


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 171