Difference between revisions of "Part:BBa J72004:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J72004 short</partinfo>
 
<partinfo>BBa_J72004 short</partinfo>
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===Source===
 
===Source===
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<pre>
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later
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PCR ca1183F/ca1183R on pG80 CRIM plasmid  (BglII/XhoI)
 +
Sub into pBca9145-Bca1144#5                (BglII/XhoI)
 +
Product is pBca9145-Bca1183
 +
----
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ca1183F BBb biobricking of phi80 att
 +
ctctgAGATCTcatgggcccgtgcgaatcag
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ca1183R BBb biobricking of phi80 att
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gcaaaCTCGAGttaGGATCCgctagctgataacgggaaag
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</pre>
  
 
===References===
 
===References===

Latest revision as of 05:32, 16 July 2008

{phi80-att} phage integration site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 411
    Illegal SpeI site found at 164
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1183F/ca1183R on pG80 CRIM plasmid   (BglII/XhoI)
Sub into pBca9145-Bca1144#5                (BglII/XhoI)
Product is pBca9145-Bca1183
----
ca1183F	BBb biobricking of phi80 att
ctctgAGATCTcatgggcccgtgcgaatcag
ca1183R	BBb biobricking of phi80 att
gcaaaCTCGAGttaGGATCCgctagctgataacgggaaag

References