Difference between revisions of "Part:BBa J72004"

Latest revision as of 05:31, 16 July 2008

{phi80-att} phage integration site

Plasmids containing the phi80 att site can be recombined into the E. coli genome with the aid of the phi80 integrase protein. Usually, this is expressed from a helper plasmid. See PMID: 11591683 for details. In house, this part is referred to as pBca9145-Bca1183 {Phi80-att}.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 164
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 411
Illegal SpeI site found at 164
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 164
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 164
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_J72004 short</partinfo>
-later
+Plasmids containing the phi80 att site can be recombined into the E. coli genome with the aid of the phi80 integrase protein.  Usually, this is expressed from a helper plasmid.  See PMID: 11591683  for details.  In house, this part is referred to as pBca9145-Bca1183	{Phi80-att}.
+----
+This part is in BBb Format.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BBb Format is available at:<br>
+[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
 <!-- Add more about the biology of this part here